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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Exosomal miR-409-3p secreted from activated mast cells promotes microglial migration, activation and neuroinflammation by targeting Nr4a2 to activate the NF-κB pathway

Fig. 2

Activated mast cells delivered exosomal miR-409-3p to murine BV-2 microglial cells. a Microarray analysis (P < 0.05, FC > 1.5) indicated that miR-409-3p was increased in LPS-P815 exosomes (LPS exo: LPS1, LPS2, LPS3; Control exo: control1, control2, control3). b Confirmation by qRT-PCR showed that miR-409-3p levels were increased in LPS-P815 cells and in the corresponding exosomes. c Murine BV-2 cells were incubated with control exosomes (Control exo) or LPS-P815 exosomes (LPS exo). The miR-409-3p levels in the two groups were detected by qRT-PCR. d Murine P815 cells were transfected with miR-409-3p mimics or mimics NC. The miR-409-3p levels in the cells and exosomes were detected by qRT-PCR. e Murine BV-2 cells were incubated with P815-miR-409-3p mimics NC-exosomes group (mimics NC exo) or P815-miR-409-3p mimics-exosomes group (mimics exo). The miR-409-3p levels were detected by qRT-PCR. F, Murine P815 cells were transfected with Cy3-labelled miR-409-3p, and exosomes were isolated from murine P815 cell culture supernatants. The isolated exosomes were labelled with PKH67 and then incubated with murine BV-2 cells. G, Fluorescence microscopy revealed that miR-409-3p could be efficiently taken up by murine BV-2 cells via exosomes. Scale bar 50 μm. Cell culture experiments were performed in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001

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