Fig. 4

miR-409-3p acted as a pro-neuroinflammatory molecule by targeting Nr4a2 to activate the NF-κB pathway. a The intersecting outputs from three prediction software programs (TargetScan, miRWalk and microT) included 30 potential target genes. Of these genes, the 3′-UTR of Nr4a2 bound to miR-409-3p with a high score and high conservation. b 3′-UTRs with Nr4a2 wild-type (WT) or mutant (Mut) binding sequences were synthesized. The luciferase reporter assay indicated that Nr4a2 was a direct target of miR-409-3p. c The protein expression levels of Nr4a2 and NF-κB/p65 were detected by western blot. d Murine BV-2 cells were co-transfected with miR-409-3p mimics and Lv-Nr4a2 (mimics + Lv-Nr4a2). The transfection efficiency and corresponding changes in NF-κB/p65 expression were also detected by western blot. E, The migratory ability of murine BV-2 cells was measured by Transwell assay. Scale bar 10 μm. F-G, The IL-6 and TNF-α levels were investigated by ELISA. Cell culture experiments were performed in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001