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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Exosomal miR-409-3p secreted from activated mast cells promotes microglial migration, activation and neuroinflammation by targeting Nr4a2 to activate the NF-κB pathway

Fig. 5

Exosome-mediated transfer of miR-409-3p promoted microglial migration, activation and neuroinflammation. a, b After murine P815 cells were transfected with miR-409-3p inhibitor or inhibitor NC, all the cells were treated with LPS. The relative miR-409-3p levels of both the cells and exosomes were determined via qRT-PCR. c Murine BV-2 cells were cultured with the corresponding exosomes extracted from these three groups (LPS exo, LPS + inhibitor NC exo, LPS + inhibitor exo). The relative miR-409-3p levels in murine BV-2 cells were detected via qRT-PCR. d The migration of murine BV-2 microglia was tested by Transwell assay. Scale bar 10 μm. e Immunofluorescence staining for Iba1 and CD68 was used to evaluate murine BV-2 microglial activation. Scale bar 50 μm. f, g The IL-6 and TNF-α levels were investigated by ELISA. H, The protein expression levels of Nr4a2 and NF-κB/p65 were detected by Western blot. Cell culture experiments were performed in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001

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