Fig. 1

Expression profile of CCL17 and CCR4 after ICH. a Representative western blot bands of time course and (b) quantitative analysis of CCL17 and CCR4 expressions, respectively after ICH; *p<0.05 vs sham, mean ± SD, one-way ANOVA, Tukey test, n=6/group. c Brain sample with schematic illustration showing the area in perihematomal region (indicated by white box) from where the images were taken for immunofluorescence staining. Representative images of co-localization of CCR4 (green) with microglia/macrophage (Iba-1, red), astrocytes (GFAP, red), and neurons (NeuN, red) in the perihematomal area at 72 h after ICH (the dotted white line shows the hematoma area). Nuclei were stained with DAPI (blue), scale bar=50 μm, n=2/group, DAPI, 4′,6-diamidino-2-phenylindole