Fig. 1

Mephedrone does not affect cell growth and viability of hBMVEC. a Treatments (96 h) with 10 μM or 100 μM mephedrone did not cause significant toxicity or cell death compared with untreated controls. Saponin (sap) was used as a positive control. Data is presented as the mean number of live cells ± SEM. Experiments were independently performed three times. Within each individual experimental set, primary cells from 3 different donors were used (n = 9). b Curves represent cell proliferation at 0 h through 120 h. Persistent exposure to 10 μM mephedrone did not change rate of cell growth. Average number of live cells was determined using Cell Viability Assay (Promega). Data is presented as the mean number of live cells ± SEM. Experiments were independently performed three times. Within each individual experimental set, primary cells from 3 different donors were used (n = 9)