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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: The psychoactive drug of abuse mephedrone differentially disrupts blood-brain barrier properties

Fig. 2

Mephedrone compromises barrier properties of hBMVEC. a Barrier electrical resistance was modelled based on continuous cell-substrate impedance readings recorded at 6 frequencies (400 Hz–48 kHz) every 6 min for the duration of the time shown. Endothelial monolayers were treated with 1 μM, 5 μM, or 10 μM mephedrone or left untreated to serve as a baseline. Treatments were initiated at 0 timepoint. Each data point is represented as the percentage of the mean value ± SEM. Experiments were independently performed three times. Within each individual experimental set, primary cells from four different donors were used (n = 12). bd Barrier permeability of small molecular tracer was modelled using FITC-conjugated dextrans (3 kDa) of various charges: neutral (b), anionic (c), and cationic (d). Endothelial monolayers were treated with 10 μM mephedrone, 100 ng/mL TNFα, or left untreated to serve as a baseline. Each data point is represented as the apparent permeability coefficient (Papp, mean value ± SEM). Experiments were independently performed three times. Within each individual experimental set, primary cells from 6 different donors were used (n = 18)

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