Fig. 4From: X chromosome escapee genes are involved in ischemic sexual dimorphism through epigenetic modification of inflammatory signalsSex differences in IRF expression after ischemia. a–e irf1, 3, 4, 5, and 8 gene mRNA levels were measured by RT-PCR in FCG mice brains. Data were averaged from 3–4 independent experiments. n=5-6 mice/group. f–i The ratio of irf4/5 gene DNA bound by histone H3K4-me1 over H3K4-me3 and H3K27-me1 over H3K27-me3 form measured by ChIP assays; the raw data are in supplementary Fig. 4. Data were averaged from 3 to 4 independent experiments with triplicate wells. *p < 0.05; **p < 0.01; ***p < 0.001Back to article page