Fig. 1

The expression of TLR4 and HMGB1 in the blood and nerve of L31 mice. a A representative western blot image showed the expression of TLR4 in the nerve of WT and L31 mice. TLR4 is significantly increased in L31 mice, with the highest increase found in L31 symptomatic mice. b A representative western blot image depicted the expression of HMGB1 in L31 mice. HMGB1 expression was increased mainly in the nerve of L31-symptomartic mice. c A representative flow cytometry histogram showed monocytes TLR4 expression in the blood. A quantitative analysis showed that TLR4 is robustly expressed in L31 mice with the highest increase found in L31-symptomatic mice. d TLR4 is expressed on blood CD8+ T cells in L31 mice with a significant increase in symptomatic mice. Quantification (c, d) depicted the number of cells per μl blood. e TLR4 is expressed on sciatic nerve macrophages in WT and L31 mice. Quantitative analysis showed a significant increase in expression in L31 symptomatic mice. Quantification (e) depicted the number of cells per a segment of 2-cm-long sciatic nerve. n = 4–5/group, *p < 0.05; **p < 0.01; ***p < 0.001