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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Inhibition of TLR4 signaling protects mice from sensory and motor dysfunction in an animal model of autoimmune peripheral neuropathy

Fig. 3

The effect of TLR4 signaling inhibition on monocyte and CD8+ T cells activation status in the blood of L31 mice. In the prevention paradigm (ae), a representative flow cytometry dot plot and quantification analysis showed a decrease in the number of CD115+CD11b+ monocytes in the blood of treated mice following TLR4 inhibition. b CCR2+ monocytes (pro-inflammatory) subset which includes CCR2+/CX3CR1 and CCR2+/CX3CR1+ were significantly reduced in treated group. c Similarly, the frequency and number of CD8+ T cells were reduced in the blood of treated mice. d The majority of the reduced CD8+ T cells in the treated group were of the activated subset (CD44+CD43+). e The frequency and number of CD8+ T cells with effector memory phenotype (CD44+CD62L) were significantly reduced in the blood of treated mice. In the reversal paradigm (fj), f frequency and total number of CD115+CD11b+ monocytes was significantly reduced in treated group. g The inflammatory subset (CCR2+CX3CR1) group was significantly reduced in treated mice as shown by representative flow and quantitative data. CCR2+/CX3CR1+ number appeared similar in both groups. h CD8+ T cells frequency and absolute number reduced in the blood following TLR4 signaling inhibition. i Majority of the reduced CD8+ T cells were of the activated (CD44+CD43+) and j effector memory phenotype (CD44+CD62L-). All quantitative analyses are shown as number of cells per μl blood. n = 6/group; *p < 0.05; **p < 0.01; ***p < 0.001

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