Fig. 4

The effect of TLR4 signaling inhibition on macrophage and CD8⁺ T cells number and activation status in the nerve of L31 mice. In the prevention paradigm (a–d), a quantitative analysis from flow cytometry showed a reduced number of macrophages (CD45⁺CD11b⁺F4/80⁺) in treated group at the end of the experiment. (b) The number of activated macrophages (MHCI⁺CD86⁺) were significantly reduced in treated group. c Frequency and absolute number of infiltrating CD8⁺ T cells were significantly diminished in treated group. In the reversal paradigm (d–f), d quantitative analysis from flow cytometry showed that TAK 242 favored the reduction of nerve macrophages (CD45⁺CD11b⁺F4/80⁺). e A quantitative analysis indicated a reduced number of activated macrophages (MHCI⁺CD86⁺). Majority of the reduced macrophages in treated group were those committed to antigen-presenting function (MHCI⁺CD86⁺). f A representative flow cytometry dot plot showed a reduced frequency and number of infiltrating CD8⁺ T cells in the sciatic nerve of treated mice. All quantitative analyses are shown as number of cells per a segment of 2-cm-long sciatic nerve. n = 6/group; *p < 0.05; **p < 0.01; ***p < 0.001