Fig. 5

CRHR1 antagonists increase synapsin I and PSD95 signals and synapsin I level in the hippocampus of the rats with prenatal DEX exposure. DEX offspring of 2 days old were subcutaneously administered with CP154526 (30mg/kg/day) or antalarmin (30mg/kg/day) for a week. The hippocampi were collected after behavioral test under anesthesia. Synapsin I (green) and PSD95 (red) were used to label presynaptic and postsynaptic terminals in hippocampus, respectively. a, b Representative fluorescence images show synapsinI-and PSD95-labeled signals in CA1 area of the hippocampus of adult female (a) and male (b) DEX offspring. c, d Histogram summarized the effects of CRHR1 antagonists on the intensity of synapsin I and PSD95-labeled signals in CA1 area of female (c) and male (d) DEX offspring. Data are presented as mean ± SEM (n=4 in each group). e&f, protein level of synapsin I in female (e) and male (f) DEX offspring was determined by western blot. Representative protein bands are presented at top of histogram. Data are presented as mean ± SEM (n=8 in each group). **P<0.01 vs control; #P<0.05, ##P<0.01 vs DEX. CTL: control, anta: antalarmin