Fig. 8

CXCL5 contributes to hippocampal CRH/CRHR1 mediated learning and memory deficits caused by prenatal DEX exposure. AAV9/CXCL5 or AAV9 was bilaterally injected into CA1 region of hippocampus of prenatal DEX. Four weeks after injection, the rats were tested for Morris water maze, and then sacrificed and hippocampus was dissected for determination of synaptic protein expression and CXCL5 concentration. a shows the injection site and immunofluorescence image of AAV9 infection in rat hippocampus. b shows CXCL5 level in DEX offspring with intra-hippocampal injection of AAV9 and AVV9/CXCL5. Data are presented as mean±SEM (n=6). *P<0.05. c-e Morris water maze tests in DEX offspring with AAV9 infusion. c&d show the escape latencies of female (c) and male (d) DEX offspring in the 4-day platform acquisition training. e shows the time that DEX offspring spent in the correct quadrant in the probe trial stage. f-i, the protein levels of synapsin I and PSD95 in hippocampus. Hippocampal tissue was obtained from adult female (f&g) and male (h&i) offspring injected with AAV9 or AAV9/CXCL5 as indicated. Representative protein bands are presented at top of histogram. Data are presented as mean ± SEM (n=6 in each group). *P<0.05 vs DEX+AAV9; **P<0.01vs control; # P<0.05, ## P<0.01 vs DEX+AAV9