Fig. 3

Effect of MPTP administration on the temporal pattern of mRNA expression of molecules involved in the brain inflammatory response. Mice were administered with saline (S) or MPTP and sacrificed at 2 h, and at 1, 2, 4 and 7 days (d) after the last MPTP injection. mRNA expression of the pro-inflammatory cytokines IL1β, IL6 and TNFα (a, b), the pro-inflammatory enzymes iNOS, COX2 and gp91phox (c, d), the anti-inflammatory cytokines IL10 and TGFβ (e, f), the anti-inflammatory markers Arg1, MR and Nrf2 (g, h) and the components of the CD200-CD200R1 inhibitory system (the ligands CD200full and CD200tr and the receptor CD200R1) (i, j) in striatum (St, left column) and ventral midbrain (MV, right column) of saline- and MPTP-treated mice. Gapdh and βactin were used as reference genes. Bars are means + SEM of seven to eight mice per experimental group. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. saline control; one-way ANOVA and Newman-Keuls post hoc test