Fig. 3

Microglial morphological phenotype does not determine cell elasticity. a Pie graphs indicating per condition the percentage of amoeboid and ramified indented microglia per brain area (WM derived = blue, GM derived = red). b Elasticity (storage modulus, upper graphs) and viscosity (loss modulus, lower graphs) of the microglia with an amoeboid or ramified phenotype derived from WM (blue) and GM (red). Black dots represent individual microglia measurements from N=6 independent cultures for control and LPS conditions and N=2 for myelin and myelin+LPS conditions. Bars represent the mean of all measurements per condition ± SEM. Representative immunocytochemical images of IBA-1 (green) in WM-derived microglia (c–f) and GM-derived microglia (g–j). Myelin phagocytosis is visualized by pHrodo (red, e, f, i, j). Arrows indicate phagocytosed myelin. Scalebar = 50 μm (c–j)