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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Viscoelastic properties of white and gray matter-derived microglia differentiate upon treatment with lipopolysaccharide but not upon treatment with myelin

Fig. 5

Semi-quantitative mRNA levels of pro-inflammatory cytokines (Il-1β, Tnfα, and Il-6) and of genes involved in antigen presentation (Hla-dr, Cd74, and B2m) in WM-derived microglia (blue, left of dotted line) and GM-derived microglia (red, right of dotted line) upon treatment with LPS, myelin, or LPS+myelin of when left untreated as a control. Data plotted is normalized against the averaged expression of two housekeeping genes (Gapdh and Hprt1) per sample per condition. Black dots represent data from 3 individual cultures; bars represent the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001

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