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Table 1 Antibodies used for immunocytochemistry

From: Viscoelastic properties of white and gray matter-derived microglia differentiate upon treatment with lipopolysaccharide but not upon treatment with myelin

Primary antibody

Dilution

Source (catalog number)

Secondary antibody

Dilution

Source

Enzymatic immunocytochemistry

 Goat x IIBA-1

1:500

Abcam (ab5076)

Impress anti-goat IgG (AP)

Undiluted

Vectorlabs

 Rabbit x GFAP

1:2000

DAKO (Z0334)

Envision+ peroxidase kit for rabbit

Undiluted

Agilent

Fluorescent immunocytochemistry

 Goat x IBA-1

1:500

Abcam (ab5076)

AF 488-labeled donkey x goat IgG

1:200

Molecular probes (A11055)

 Rhodamine phalloidine (F-actin)

1:80

Life Technologies (R415)

NA

NA

NA

  1. F-actin-binding rhodamine phalloidine is fluorescently labeled
  2. NA not applicable