Primary antibody | Dilution | Source (catalog number) | Secondary antibody | Dilution | Source |
---|---|---|---|---|---|
Enzymatic immunocytochemistry | |||||
Goat x IIBA-1 | 1:500 | Abcam (ab5076) | Impress anti-goat IgG (AP) | Undiluted | Vectorlabs |
Rabbit x GFAP | 1:2000 | DAKO (Z0334) | Envision+ peroxidase kit for rabbit | Undiluted | Agilent |
Fluorescent immunocytochemistry | |||||
Goat x IBA-1 | 1:500 | Abcam (ab5076) | AF 488-labeled donkey x goat IgG | 1:200 | Molecular probes (A11055) |
Rhodamine phalloidine (F-actin) | 1:80 | Life Technologies (R415) | NA | NA | NA |