Fig. 1

Development of Btg2^−/− mice by genome editing. a Target sequence is shown. Black arrows show the primer pair used for genotyping, and white arrows show the primer pairs used for real-time PCR. PAM, protospacer-adjacent motif. b Genomic sequence of wild-type mice (upper) and Btg2^−/− mice (bottom). Eleven bases were deleted from exon I of the mouse Btg2 gene. c Genotyping pattern of wild-type (+/+), heterozygous deletion (+/−), and homozygous deletion (−/−) of Btg2, separated on a 6% acrylamide gel. d RT-PCR products of total brain RNA from wild-type (+/+) and homozygous deletion of Btg2 (−/−), separated on a 2% agarose gel. β-actin was used as a loading control