Fig. 5

Effect of 4R on STAT3, Akt1, and CREB phosphorylation in WT and α7KO mice. Each graph represents the phosphorylated form normalized by total protein. a–c Hippocampus median fluorescence intensity (MFI) in both WT and α7KO mice. d–f Cortex MFI in both mouse strains. a, d pSTAT3 at Tyr 705. b, e pAkt1 at Ser 473. c, f pCREB at Ser 133. g–n Protein expression of pSTAT3, pAkt1, and pCREB in WT mice analyzed by Western blot in the hippocampus (g–j), and cortex (k–n) at the same phosphorylation sites. a–f Two-way ANOVA followed by Tukey’s post hoc test. g–n One-way ANOVA followed by Tukey’s post hoc test. *p < 0.05, **p < 0.01 versus saline; ^#p < 0.05, ^##p < 0.01, ^####p < 0.0001 versus LPS/sal; ^†p < 0.05, ^††p < 0.01, ^†††p < 0.001, ^††††p < 0.0001, versus LPS/veh; ^ττττp < 0.0001, LPS/4R WT versus LPS/4R α7KO mice. Data presented as mean ± SEM. n = 5 mice per group in duplicate, for Western blot n = 3 per group