Fig. 4

Oxaliplatin activates the NF-κB signaling pathway in the spinal dorsal horn. Rats were treated with vehicle or oxaliplatin (0.4 mg/100 g/day for five consecutive days), and the L4–L6 dorsal horns were harvested for the following examines. a Protein levels of IκB-α. GAPDH served as loading controls. **p < 0.01; n = 4. Protein levels of NF-κB P65 in the nucleus (b) and in the cytoplasm (c). H3 and GAPDH served as loading controls for nuclear and cytoplasmic proteins. *p < 0.05; n = 4. d IP with IκB-α antibody followed by immunoblotting using P65 antibody. e Transcriptional levels of IL-1β, TNF-α, and IL-6. *p < 0.05 and **p < 0.01; n = 4. Oxal, oxaliplatin