Fig. 3

Subarachnoidal and intraparenchymal infiltration in cerebellum of EAE-induced GF-IL23 mice. HE- and LFB-staining of the cerebellum at different time points (d0, d15, d22, d33, d60) after induction of the EAE. Naïve GF-IL23 mice show no infiltration (a higher magnification (d), WT controlls (g, j)). GF-IL23 mice (b, c, m, n, o higher magnification (e, f, p, q, r)) show increasing subarachnoidal (arrow) and intraparenchymal (arrow head) infiltrates over the time and persistent demyelination on day 60 whereas WT mice (h, i, s, t higher magnification (k, l, v, w)) show only little and few infiltrates without persisting demyelination on day 60. Scale bar: 100 μm. The images are representative of those from at least 8 different transgenic/WT mice d0, d15, d22, d33, and 8 transgenic, 6 WT mice d60