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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Inhibition of MLKL-dependent necroptosis via downregulating interleukin-1R1 contributes to neuroprotection of hypoxic preconditioning in transient global cerebral ischemic rats

Fig. 5

Hypoxic preconditioning downregulates the expression of IL-1R1 and alleviates p-MLKL-dependent neuronal damage in CA1 after tGCI. A Western blot analysis of IL-1R1 in CA1. The histogram presents the quantitative analyses of IL-1R1 levels. B Immunoprecipitation and western blot assays showing the effects of hypoxic preconditioning on the formation of IL-1R1-p-MLKL (B, a) and the expression of IL-1R1 and p-MLKL (B, b) in CA1 of tGCI. C Representative images of western blotting showing the expression of IL-1R1 in CA1 after tGCI with or without IL-1RA administration. D Immunoprecipitation and western blot assays showing the effects of IL-1RA on the formation of IL-1R1-p-MLKL (D, a) and the expression of IL-1R1 and p-MLKL (D, b) in CA1 of tGCI. E Effects of pretreatment with IL-1RA on the p-MLKL expression in plasma membrane protein in CA1 of tGCI rats with or without hypoxia using immunoblot analysis. F Immunoprecipitation and western blot assays showing the effects of IL-1RA on the formation of RIP3-p-MLKL (F, a) and the expression of RIP3 and p-MLKL (F, b) in CA1 of tGCI. G Representative microphotographs of cresyl violet staining, immunostaining of NeuN and FJ-B staining in the hippocampus at 7 days after tGCI with or without IL-1RA administration. H–J Quantitative analyses of surviving cells, NeuN and FJ-B-positive cells in CA1. Each bar represents the mean±S.D. *p<0.05 vs. Sham+Vehicle animals, #p<0.05 vs. tGCI+Vehicle group and &p<0.05 vs. tGCI or HPC group administrated with vehicle. IP immunoprecipitation, IB immunoblotting, HPC hypoxic preconditioning

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Journal of Neuroinflammation

ISSN: 1742-2094