Fig. 5

In situ measurement of MBP and NF protein densities showed no significant change. a–c MBP immunostained myelinated nerve fibers in saline cases (a), LPS group (b), and LPS + minocycline animals (c). d Statistical comparison of measured MBP immunofluorescence intensity exhibits no apparent difference between the saline, LPS, and LPS + minocycline groups (N = 3 in the saline and LPS groups, N = 4 in the LPS + minocycline rats). e–g NF immunoreactivity of CC nerve fibers in the saline (e), system LPS (f), and LPS + minocycline groups (g). h Statistical process of measured NF immunofluorescence intensity shows no significant difference between the 3 groups (N = 3 in the saline and LPS groups, N = 4 in the LPS + minocycline rats); however, NF intensity of immunofluorescent labeling in the LPS group was obviously lower than that in the saline and LPS + minocycline groups. Scale bars for a–c and e–g are 50 μm