Fig. 4

4-EG represses MG activation in EAE. a At day 12–13 post-immunization, mononuclear cells were isolated from the brain and spinal cord of vehicle- and 4-EG (100 mg/kg)-treated C57BL/6 EAE mice followed by flow cytometry analysis to determine CD80 and CD86 expression on CD45^intCD11b⁺ MG (n=5/group). Isotype controls (Iso) were used as a negative control to determine MG positive for CD80 or CD86 expression. Data are representative of two independent experiments (n=5/group per experiment). b The lumbar regions of spinal cord tissues were collected from vehicle- and 4-EG-treated C57BL/6 EAE mice (n=7/group) followed by immunofluorescence analysis to determine cells positive for Iba1 expression. The number of Iba1⁺ cells was calculated and quantified under ×20 magnification. Scale bars, 200 μm (×10, left image), 50 μm (×20, right image). Statistical significance was determined as ***p<0.001 by unpaired t test