Fig. 2
From: STEAP4 expression in CNS resident cells promotes Th17 cell-induced autoimmune encephalomyelitis
STEAP4−/− T cells are fully encephalitogenic. Mice were immunized by MOG35–55 and sacrificed 10 days after immunization. Lymph node cells were harvested and re-stimulated with MOG35–55 in DMEM medium alone (Th0), 20 ng/ml IL-12 (Th1), or 20 ng/ml IL-23 (Th17) for 5 days. a Data show the frequency of Th1 or Th17 cells under each polarization by intracellular staining. b Draining lymph node cells from wild-type mice and STEAP4-deficient mice 10 days after immunization were re-stimulated with MOG35–55 in vitro for 5 days, followed by ELISA of IL-17, IFN-γ. c MOG35–55-specific Th17 cells from wild-type mice and STEAP4-deficient mice were used as donor cells and transferred to naïve wild-type recipient mice. Graph represents the mean clinical score after Th17-cell transfer. d H&E and myelin basic protein (MBP) staining of lumber spinal cords at the end point of EAE. e Absolute cell numbers of infiltrated immune cells in the brains of recipients of STEAP4+/- Th17 or recipients of STEAP4−/− Th17 cells. Data are representative of three independent experiments. n = 5/group in each experiment. Error bars, SEM; *p < 0.05, **p < 0.01