Fig. 3
From: STEAP4 expression in CNS resident cells promotes Th17 cell-induced autoimmune encephalomyelitis
Ablation of STEAP4 ameliorates Th17 cell-induced but not Th1 cell-induced EAE. a Microarray analysis of STEAP4 expression in spinal cords from wild-type B6 mice that received MOG35–55-reactive Th1 cells or Th17 cells and at the peak of disease, relative to the naïve control mice. Data are re-plotted from GSE97035. b MOG35–55-specific Th17 cells from wild-type mice were used as donor cells and transferred to naïve STEAP4-deficient mice and the littermate wild-type controls. Graph represents the mean clinical score after Th17-cell transfer. c H&E and myelin basic protein (MBP) staining of lumber spinal cords at the end point of EAE. d Absolute cell numbers of infiltrated immune cells in the brains of STEAP4+/− and STEAP4+/− recipient mice at the end point of EAE. e Real-time PCR analysis of inflammatory gene expression in spinal cords of STEAP4+/− and STEAP4+/− mice transferred with MOG35–55-specific Th17 cells. f MOG35–55-specific Th1 cells from wild-type mice were used as donor cells and transferred to naïve STEAP4-deficient mice and the littermate wild-type controls. Graph represents the mean clinical score after Th1-cell transfer. g H&E and MBP staining of the spinal cords from wild-type and STEAP4-deficient mice transferred with Th1 cells at the end point of Th1 EAE. h Immune cell infiltration in the brains of wild-type and STEAP4-deficient mice at the end points of Th1 EAE analyzed by flow cytometry. Data are representative of three independent experiments. n = 5/group in each experiment. Error bars, SEM; *p < 0.05, **p < 0.01, ***p < 0.001