Fig. 5
From: STEAP4 expression in CNS resident cells promotes Th17 cell-induced autoimmune encephalomyelitis
Ablation of STEAP4 in neuroectoderm-derived cells ameliorates Th17 cell-induced EAE.
a Mean clinical score of EAE in Nestin-Cre STEAP4fl/+ and Nestin-Cre Steap4fl/fl mice after MOG35–55-specific Th17 cell adoptive transfer. b H&E and myelin basic protein (MBP) staining of lumber spinal cords at the end point of EAE. c Absolute cell numbers of infiltrated immune cells in the brains of STEAP4+/− and STEAP4+/− recipient mice at the end point of EAE. d Real-time PCR analysis of inflammatory gene expression in spinal cords of Steap4+/− and STEAP4+/− mice transferred with MOG35–55-specific Th17 cells. e MOG35–55-specific Th1 cells from wild-type mice were used as donor cells and transferred to naïve STEAP4-deficient mice and the littermate wild-type controls. Graph represents the mean clinical score of EAE after Th1-cell transfer. f H&E and MBP staining of the spinal cords from Nestin-Cre STEAP4fl/+ and Nestin-Cre STEAP4fl/fl mice at the end point of EAE. g Immune cell infiltration in the brains of Nestin-Cre STEAP4fl/+ and Nestin-Cre STEAP4fl/fl mice at the end point of EAE analyzed by flow cytometry. Data are representative of three independent experiments. n = 5/group in each experiment. Error bars, SEM; *p < 0.05, **p < 0.01