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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Human Huntington’s disease pluripotent stem cell-derived microglia develop normally but are abnormally hyper-reactive and release elevated levels of reactive oxygen species

Fig. 2

Human Huntington’s disease PSC-derived microglia are hyper-reactive to an immune challenge. IsoHD PSC-derived microglia were stimulated with 1 μg/ml LPS and 10 ng/ml IFNγ for 24 h and their supernatants assessed by multiplex ELISA for the secretion of inflammation-associated cytokines. In most cases, there was a trend of increased production of the cytokines measured, with statistically significantly elevated levels of the pro-inflammatory cytokines, IL-6 and TNFα. Data are expressed as the fold-change following stimulation normalised to total cell number, presented as mean ± SEM, n = 3, and analysed by one-way ANOVA with Tukey’s post-test, (* p < 0.05)

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