Fig. 3
Human Huntington’s disease PSC-derived microglia show elevated production of reactive oxygen species. a IsoHD PSC-derived microglia were assessed by DCFDA/H2DCFDA assay for the steady-state production of ROS. This showed statistically significantly increased levels of ROS production by microglia expressing HTT with pathogenic polyglutamine repeats compared with the non-pathogenic, 30Q control. Data are presented as mean ± SEM, n = 3, analysed by one-way ANOVA with Tukey’s post-test (*p < 0.05). b IsoHD and other PSC-derived microglia were then assessed for production of ROS with and without treatment with 110 μM TBHP, a ROS-inducing toxin, for 4 h. Under non-treated, baseline conditions, linear regression analysis of all the lines did not show a significant HTT polyglutamine-dependent effect on ROS production. However, under the TBHP-treated condition, a HTT polyglutamine-dependent effect was observed, as shown by a linear regression slope that was significantly non-zero. Data are presented as mean, ± SEM where multiple measurements of the same line were made. The identity of the lines used are as follows: circles = lines of the IsoHD ESC series, triangles = lines of the HD family iPSC series, square = 1534 iPSC line, hexagon = QS5.1 line