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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Human Huntington’s disease pluripotent stem cell-derived microglia develop normally but are abnormally hyper-reactive and release elevated levels of reactive oxygen species

Fig. 5

Human PSC-derived microglia show elevated cell death when treated with media from human PSC-derived striatal neurons expressing mutant HTT with a long polyglutamine-tract. IsoHD PSC-derived microglia were cultured with supernatants harvested from IsoHD PSC-derived striatal neurons. a Exposure to supernatants from untreated neuronal cultures for five days did not reveal any HTT polyglutamine-dependent effects, either of the donor neurons or the recipient microglia, on LDH release by the microglia. b By contrast, when the neurons had been heat-shocked at 45 °C for 45 min to induce stress, recipient microglia of all HTT genotypes showed elevated LDH release when treated with supernatants from HTT 80Q neurons compared with those from the 30Q and 45Q ones. Data are presented as mean ± SEM, n = 3, analysed by two-way ANOVA with Tukey’s multiple comparisons test

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Journal of Neuroinflammation

ISSN: 1742-2094