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Fig. 6. | Journal of Neuroinflammation

Fig. 6.

From: Human Huntington’s disease pluripotent stem cell-derived microglia develop normally but are abnormally hyper-reactive and release elevated levels of reactive oxygen species

Fig. 6.

Human Huntington’s disease PSC-derived microglia conditioned media are not toxic to human PSC-derived striatal neurons. IsoHD PSC-derived striatal neurons were cultured with supernatants harvested from IsoHD PSC-derived microglia. a, b Numbers of recipient cells demonstrating caspase 3 activation as a marker of apoptosis were not affected by exposure for five days to conditioned media from microglia expressing mutant HTT, when either a the microglia were unstimulated or b the microglia had been activated by treatment with 1 μg/ml LPS and 10 ng/ml IFNγ for 24 h. c, d Numbers of recipient cells showing increased numbers of γH2ax foci, a marker of DNA damage and genotoxic stress, were lower in HTT 45Q and 81Q neurons than 30Q ones, irrespective of whether they had been treated or not with conditioned media from either c unstimulated or d LPS-stimulated mutant HTT-expressing microglia. Data are presented as mean ± SEM, n = 3, analysed by two-way ANOVA with Tukey’s multiple comparisons test

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