Fig. 6

TrkB.T1 receptor contributed to RTX-PHN through ASIC3 signaling. a 7,8-DHF decreased the paw withdrawal threshold of healthy rats (n = 10; ^∆∆p < 0.01, control vs. 1 mg DHF; **p < 0.01, control vs. 3mg DHF; ^&&p < 0.01, control vs. 6 mg DHF; ^#p <0.05, ^##p <0.01, 1mg DHF vs. 3mg DHF; two-way repeated measures ANOVA followed by Bonferroni’s post hoc test). b 7,8-DHF and amiloride treatment reversed the decreased paw withdrawal threshold (n = 10; *p < 0.05, **p < 0.01 vs. RTX-PHN; two-way repeated measures ANOVA followed by Bonferroni’s post hoc test). c–f 7,8-DHF and Amiloride treatment inhibited protein expression of TRAF6, nNOS and c-Fos (n = 4, *p < 0.05, **p < 0.01 vs. RTX-PHN; Student’s t-test)