Fig. 9

Knockdown of ASCI3 abolished TRAF6 signaling activation induced by exogenous BDNF treatment in PC-12 cells. a, b Transfection with shRNAs reduced ASIC3 protein levels (n = 3, *p < 0.05, **p < 0.01 vs. NC group; ^∆∆p < 0.01 vs. shRNA2 group; ^#p < 0.05 vs. shRNA1 group; one-way ANOVA followed by Tukey’s post hoc test). c, d ASIC3 protein levels in NC group was not changed compared with the control group (n = 3). e, f shRNA transfection inhibited the protein expressions of ASIC3, TRAF6 and nNOS induced by exogenous BDNF(n = 3). g Exogenous BDNF increased NO level, which was reversed by shRNA transfection(n = 5). h–j Exogenous BDNF upregulated mRNA expressions of IL-6, IL-1β and TNF-α, which was reversed by shRNA transfection(n = 5). *p < 0.05, **p < 0.01 vs. control group; ^#p < 0.05, ^##p < 0.01 vs. BDNF group; one-way ANOVA followed by Tukey’s post hoc test