Fig. 4
mRNA expression levels of foxp3, TGFβ, GATA3, T-bet, and RORγt following GAT107 treatment. Mice were treated with GAT107 (10 mg/day, i.p.) for 8 days from the day of EAE induction. One day later, pooled lymphocytes were obtained and activated with MOG35–55 as described in the “Methods”. mRNA levels of foxp3, TGFβ, GATA3, T-bet, and RORγt were assessed by RT-PCR. The results are expressed as mean relative quantification value ± SE of three separate experiments (n = 6–7 in each group) (*p < 0.05)