Fig. 4

Evaluation of neurobehavioral abilities of POCD mice after inhibition of CX3CR1 signaling (one-way ANOVA with Tukey’s post hoc, n = 5~13 per group). a Latency to enter the dark compartment in the passive avoidance test 2 days after tibial fracture surgery. b Leaning index (transfer latency time to enter the closed arm of the training period − transfer latency time to enter the closed arm during the test period) in the elevated plus maze test. c Discrimination time rate for novel objects in the test phase of the novel objective recognition test. d Time rate in the center zone in the open-field test. e Mechanical allodynia was evaluated with the von Frey test. Control, normal control group; TF2D, 2 days after tibial fracture surgery group; TF+Ab, 2 days after tibial fracture surgery group injected with neutralizing Ab; Control+Ab, normal control group injected with neutralizing Ab; *p < 0.05 compared to the control; ^#p < 0.05 compared to the TF2D; ^###p < 0.0001 compared to TF2D