Fig. 7
From: Orthopedic surgery-induced cognitive dysfunction is mediated by CX3CL1/R1 signaling
Effect of inhibition of CX3CR1 signaling on GFAP, Mao B expression, and GABA levels. a Representative immunofluorescence images for DAPI, GFAP, and Mao B. b Sholl analysis for a traced individual GFAP-positive astrocyte, which is superimposed over concentric circles (one-way ANOVA with Tukey’s post hoc test, n = 8 per group). c Intensity of Mao B images. d Quantification of the total number of intercepts with ramifications from Sholl analysis (one-way ANOVA with Tukey’s post hoc test, n = 10 per group). a Representative immunofluorescence images for DAPI and GABA. b Quantification of GABA-positive cells (one-way ANOVA with Tukey’s post hoc test, n = 8 per group). Control, normal control group; TF_Veh, 2 days after tibial fracture surgery group injected with saline; TF_Ab, 2 days after tibial fracture surgery group injected with neutralizing Ab; Control+Ab, normal control group injected with neutralizing Ab; scale bar, 20 μm, 100 μm. *p < 0.05; **p < 0.01; ***p < 0.0001 compared to the control; #p < 0.05; ##p < 0.01; ###p < 0.0001 compared to TF2D