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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: TNF-α acutely enhances acid-sensing ion channel currents in rat dorsal root ganglion neurons via a p38 MAPK pathway

Fig. 2

TNF-α shifted upwards the concentration-response curve for protons. a Sequential currents were evoked by three different low pH values in the absence and presence of 10 ng/ml TNF-α pretreatment. b Concentration-response curves for protons in the absence (, white circle) and presence (, black circle) of TNF-α. Concentration-response curve for protons shifted upwards in the presence of extracellular TNF-α (10 ng/ml). Each point represents the mean ± S.E.M. of 7–10 DRG neurons from 4–6 rats. All peak current values were normalized to the peak current maximally activated by pH 4.5 applied alone in the absence of TNF-α (marked with asterisk). The figure shows averaged data fitted with the Hill equation. The curves shown are a best fit of the data to the logistic equation I = Imax/[1 + (pH0.5/pH)n], where pH is the pH value used, I is the normalized current response value, pH0.5 is the pH value for half-maximal current response, and n is the Hill coefficient. The Hill coefficients for the cases with and without TNF-α pre-application were 1.32 and 1.30, respectively

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