Fig. 3
p38 MAPK, but not COX, was required for TNF-α-induced enhancement of ASIC currents. Representative current traces in (a) and the bar graph in (b) showed that the effects of TNF-α (10 ng/ml) alone, p38 MAPK inhibitor SB202190 plus TNF-α, and non-selective COX inhibitor indomethacin plus TNF-α on pH 6.0 acid- induced currents. IpH6.0 was enhanced by TNF-α (10 ng/ml) pre-applied alone for 5 min, and the TNF-α (10 ng/ml) enhancement of IpH6.0 was blocked by the pre-treatment of SB202190 (10 μM, 3+5=8 min), but not by the pre-treatment of indomethacin (30 μM, 3+5=8 min). Statistical tests were performed using one-way ANOVA followed by post hoc Bonferroni’s test, and significance is shown **P < 0.01. n.s. Not significant. n = 6 in each column