Fig. 5
From: Conditional knockout of Tsc1 in RORγt-expressing cells induces brain damage and early death in mice
Abnormal activation of neurons and astrogliosis in the cortex and hippocampus of Tsc1RORγt mice. a–d Representative images of Nissl staining of the cerebral cortex (a) and different regions of the hippocampus (b–d) of Tsc1RORγt mice and control littermates. e Statistical analysis of the area fraction of Nissl bodies shown in a–d. f–h Representative images of GFAP immunostaining (green) (f and j) and the area fraction of GFAP+ astrocytes and NeuN+ neurons (h) in the cerebral cortex (f) and hippocampus (b) of Tsc1RORγt mice and control littermates. Each dot represents one mouse and the error bars represent SEMs; *p < 0.05 and **p < 0.01. Unpaired two-tailed Student’s t test (e, f, g). The locations of the magnified box are identified in the original image. All images were captured from scans of whole-brain slices. Magnification: 10x (a, f) and × 40 (b, c, d, g). Scale bars: 50 μm (b, c, d, g) and 100 μm (a, f)