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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Oxidative stress induced by NOX2 contributes to neuropathic pain via plasma membrane translocation of PKCε in rat dorsal root ganglion neurons

Fig. 5

Pretreatment with gp91-tat prevents SNI-induced plasma membrane translocation of PKCε in DRG neurons. a Representative double-immunofluorescence staining showing the colocalization of PKCε with IB4 (a(a–c)), CGRP (a(d–f)), and NF-200 (a(g–i)) but not GFAP (a(j–l)) in the sham, SNI, and SNI pretreated with gp91-tat groups (n = 3/group). b Representative staining showing the plasma membrane translocation of PKCε in the SNI group (b(c, d)) but not in the sham (b(a, b)) and gp91-tat-pretreated groups (b(e, f)) presented as the fluorescence intensity of PKCε in the cell. c, d PKCε expression in both the plasma membrane (c) and cytosolic fractions (d) of DRGs from the sham, SNI, and gp91-tat-pretreated SNI groups was determined by Western blotting (n = 5/group). One-way ANOVA followed by Tukey’s test. *p < 0.05, **p < 0.01 versus the sham group; #p < 0.05, ##p < 0.01 versus the SNI group

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