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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Oxidative stress induced by NOX2 contributes to neuropathic pain via plasma membrane translocation of PKCε in rat dorsal root ganglion neurons

Fig. 6

H2O2 induces the plasma membrane translocation of PKCε. a Experimental diagram showing the timeline of H2O2 application, immunohistochemistry, and Western blot analysis. b, c Western blot showing that PKCε protein expression was increased in both plasma membrane (b) and cytosol (c) of DRGs after the local application of H2O2 (n = 5/group). Two-tailed unpaired Student’s t test. **p < 0.01 versus the vehicle group. d Representative staining illustrating the plasma membrane translocation of PKCε in DRG sections after 1 and 4 days of local H2O2 administration. The fluorescence intensity of PKCε in the cell (indicated by the arrow) is illustrated. e Representative staining showing that PKCε translocated to the plasma membrane in cultured L4-L6 DRG neurons after 1 and 4 days of H2O2 treatment. The fluorescence intensity of PKCε in the cell (indicated by the arrow) is illustrated. f Ratios of the PKCε fluorescence intensity in the membrane and total soma of control cells and at 1 day and 4 days after drug application (n = 32–48/group). One-way ANOVA followed by Dunnett's T3 multiple comparisons test. ***p < 0.001 versus the control group. g Quantitative measurements showing the fluorescence intensity ratios of membrane and total soma in small, medium, and large DRGs (n = 12–25/group). One-way ANOVA followed by Dunnett's T3 multiple comparisons test. ***p < 0.001 versus the control group

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