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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Acute inflammatory profiles differ with sex and age after spinal cord injury

Fig. 4

Activation-, ROS-, and debris-associated gene expression differs between MDMs and microglia at 3-DPI. MDMs and microglia were FACS sorted into separate tubes at 3-DPI, and RNA was isolated. RNA expression was quantified in up to 27 genes associated with activation, ROS, and debris response. a Genes known to be regulated specifically in MDMs or microglia were assessed to determine the purity and efficiency of FACS sorting and were analyzed using T test. All other analyses of RNA counts were assessed using a one-way MANOVA to determine the main effects between MDMs and microglia. Mean values were analyzed first by T test then by the two-stage step-up method of Benjamini, Krieger, and Yekutieli for FDR adjustment to control for multiple comparisons. c All but 3 genes were differentially regulated between cell types (p<0.05, C); SOCS3 (p=0.47), STAT1 (p=0.25), and TNF (p=0.36) were not different between MDMs and microglia. b All genes that were differentially regulated between cells were upregulated in MDMs with three exceptions; C1qa (p<0.001, q<0.001), CD86 (p<0.001, q<0.001), and cPLA2 (p<0.001, q<0.001) were upregulated in microglia. Sample size include MDMs (n=15) and microglia (n=14). All graphs present SEM. *Significant T test at least at p<0.05, qsignificant discovery after FDR at least at q<0.05

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