Fig. 5

Activation, ROS, and debris-associated gene expression differs by sex in a cell-dependent manner at 3-DPI. MDMs and microglia were FACS sorted into separate tubes at 3-DPI, and RNA was isolated. RNA expression was quantified in up to 27 genes associated with activation, ROS, and debris response. RNA counts were assessed using a two-way MANOVA to determine the main effects between sex and age within each cell type. Because no interactions were found, the estimated marginal means with corresponding SEM’s were used to perform T tests and FDR analyses to correct for the combined variable. a A main effect of sex was found in MDMs (p<0.05), with 4 genes being significant at the level of T test, and only 1 gene persevering after FDR correction. a Specifically, CD80 (p<0.01, q=0.056), STAT6 (p<0.05, q=0.27), and HIF1α (p<0.05, q=0.13) were significantly increased in female MDMs, with C1qa (p<0.001, q<0.01) being upregulated in male MDMs as a significant discovery. No significant effects were found for age in MDMs. b Of 5 genes upregulated in female microglia, 2 were found to be significant discoveries. b Specifically, CD80 (p<0.05, q=0.22), Arg1 (p<0.05, q=0.19), and SOD1(p<0.05, q=0.22) were significantly increased in female microglia, with CYBB (p<0.01, q<0.05), and MRC1 (p<0.01, q<0.05), being significant discoveries after FDR correction. c Only two genes were significantly affected by age: STAT6 being decreased with age (p<0.05, q=0.30) and CYBB being increased with age (p<0.05, q=0.30). Sample size include females (MDMs, n=7; microglia, n=6), males (MDMs, n=8; microglia, n=8), 4-MO (MDMs, n=6; microglia, n=6), 14-MO (MDMs, n=9; microglia, n=8). All graphs present SEM. *Significant T test at least at p<0.05, ^qsignificant discovery after FDR at least at q<0.05