Fig. 1
From: Capsazepine decreases corneal pain syndrome in severe dry eye disease
Evaluation of nociceptor expression in the ophthalmic branch of the trigeminal ganglion (TG) of sham and dry eye disease (DED) animals. a Localization of the ophthalmic branch (V1, red rectangle), maxillary branch (V2), and mandibular branch (V3) in a mouse TG using the positive probe of RNAscope (Opal 520). Staining (white arrows) and quantification of mRNA levels by in situ hybridization of (b) transient receptor potential vanilloid-1 (TRPV1; Opal 520), (c) transient receptor potential ankyrin 1 (TRPA1; Opal 570), (d) acid-sensing ion channel 1 (ASIC1; Opal 570), (e) ASIC3 (Opal 650), and (f) Piezo-2 (Opal 650) in the ophthalmic branch of the TG of sham and DED animals. All experiments were conducted on d21. Total number of mice = 44. Sham and DED animals n = 4–6. Scale bar = 50 μM. *P < 0.05 relative to sham group. Results are expressed as the mean ± SEM. For statistical analysis, the Kolmogorov–Smirnov test was performed followed by a parametric t-test (TRPV1) or nonparametric Mann-Whitney test (TRPA1, ASIC1, ASIC3, and Piezo-2) using GraphPad Prism version 7.00 (GraphPad Software, La Jolla, CA, USA)