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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Complete spatial characterisation of N-glycosylation upon striatal neuroinflammation in the rodent brain

Fig. 2

Pathological validation of the model of striatal neuroinflammation where LPS was injected into the striatum, in comparison to the contralateral (non-injected (NI)) striatum at seven days post-injection (dpi). a In vivo analysis of translocator protein (TSPO) expression as a marker of neuroinflammation in the striatum post-LPS injection. Average quantified TSPO-PET image in non-displaceable binding potential (BPND) in a coronal section. b Quantification of TSPO-PET imaging in the striatum. Results are expressed as means ± the standard error of the mean (SEM). n = 9; paired Student t test and statistically significant difference was set at **p < 0.01. c Striatal mRNA expression of different genes related to the inflammatory response–Glial fibrillary protein (GFAP), Iba1, vimentin (Vim), TSPO and tumour necrosis factor α (TNFα). Results are expressed as means ± SEM. n = 11–12; paired Student t test and statistically significant difference was set at ****p < 0.0001. d Spearman correlation between in vivo individual PET BPnd data and post-mortem expression data. Spearman correlation coefficients are marked in the corresponding case; blue signifies a positive correlation. Statistical significance was set at *p < 0.01. e, f, g Histological evaluation of the expression of Iba1, GFAP and Vim (respectively) in LPS-injected vs non-injected striata at seven dpi. Scale bar = 50 μm. h, i, j Striatal optical density of Iba+, GFAP+ or Vim+ (respectively) in the LPS-injected and NI striata. Results are expressed as means ± SEM. n = 10–12; paired Student t test and statistically significant difference was set at ***p < 0.001, and ****p < 0.0001

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