Table 1 List of primers used for RT-qPCR
Gene/Primer | Forward sequence | Reverse sequence |
|---|---|---|
Ppia/Cyclo (housekeeping) | ATGGCAAATGCTGGACCAAA | GCCTTCTTTCACCTTCCCAAA |
Rplp0 (housekeeping) | CAGGCGTCCTCATTAGAG | ATCTGCTGCATCTGCTTGGAG |
Hprt1 (housekeeping) | GGACCTCTCGAAGTGTTGGATAC | CCCTGAAGTGCTCATTATAGTCAA |
GFAP | AATGACTATCGCCGCCAAC | CTCCTGGTAACTCGCCGACT |
IBA1 | CCAGCCTAAGACAACCAGCGTC | GCTGTATTTGGGATCATCGAGGAA |
TNFα | AAATGGGCTCCCTCTCATCAGTTC | TCTGCTTGGTGGTTTGCTACGAC |
TSPO/PBR | CAGTGTCCTTCACGGAGCAG | CGGGTACCCAGGATTGAGAC |
Vimentin | GCAAAGCAGGAGTCAAACGA | AATTCTCTTCCATTTCACGCATCT |