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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: High-fat diet impairs duodenal barrier function and elicits glia-dependent changes along the gut-brain axis that are required for anxiogenic and depressive-like behaviors

Fig. 4

HFD drives glial-dependent changes in the hippocampus and neuronal dendritic spines decrease. Representative images of hippocampal slices from mice fed with a standard diet (SD) or high-fat diet (HFD) for 20 weeks, in the absence or presence of daily IP of 10 μmol/kg fluorocitrate (FC). Triple-label immunofluorescence for GFAP (green), TLR4 (red), and Dapi (blue) and related fluorescence intensity quantification at weeks 6 and 20 (ac) of diet protocol are shown, respectively. In parallel, BDNF protein expression (green) in the dentate gyrus was assessed at 6 and 20 weeks (d and e), respectively. The number of dendritic spines (PSD 95 immunoreactivity, red) was measured in hippocampal neurons’ dendrites (MAP-2 immunoreactivity, green) isolated from the abovementioned experimental groups at weeks 6 (f and g) and 20 (f and h) of diet protocol. Data were analyzed by 2-way ANOVA and 1-way ANOVA and Dunnett post hoc. Results are expressed as average relative fluorescence units (RFU) ± SEM per area unit or the average number of dendritic spines/10 μm of n assessments. *P<0.05, **P<0.01, and ****P<0.0001 versus relative SD group. Scale bars = 10 and 20 μm

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