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Table 2 Primary and secondary antibodies used for immunofluorescence and immunohistochemistry labeling

From: High-fat diet impairs duodenal barrier function and elicits glia-dependent changes along the gut-brain axis that are required for anxiogenic and depressive-like behaviors

Antigen Host Dilution Source Cat# / RRID
ZO-1 Rabbit 1:150 Bioss bs-1329R
Occludin Mouse 1:100 Invitrogen - Thermofisher scientific 33-1500
CD 45 Rabbit 1:500 Abcam Ab10558
GFAP Rabbit 1:500 ATLAS HPA056030
TLR4 Mouse 1:250 Invitrogen - Thermofisher scientific MA5-16216
BDNF Rabbit 1:200 Bioss BS-4989R
HuCD Mouse 1:15 Invitrogen - Thermofisher scientific A-21271
MAP-2 Rabbit 1:1000 Abcam ab32454
PSD 95 Mouse 1:200 Invitrogen 7E3-1B8
Anti-Rabbit IgG (H+L) [FITC] Donkey 1:1000 Novusbio NB120-6798
Biotin-SP (long spacer) AffiniPure F(ab')2 Fragment Anti-Rabbit IgG (H+L) Donkey 1:3000 Jackson ImmunoResearch 711-066-152
Anti-Mouse IgG (H+L) [Texas Red] Goat 1:500 Novusbio NB120-6787