Table 2 Primary and secondary antibodies used for immunofluorescence and immunohistochemistry labeling
Antigen | Host | Dilution | Source | Cat# / RRID |
|---|---|---|---|---|
ZO-1 | Rabbit | 1:150 | Bioss | bs-1329R |
Occludin | Mouse | 1:100 | Invitrogen - Thermofisher scientific | 33-1500 |
CD 45 | Rabbit | 1:500 | Abcam | Ab10558 |
GFAP | Rabbit | 1:500 | ATLAS | HPA056030 |
TLR4 | Mouse | 1:250 | Invitrogen - Thermofisher scientific | MA5-16216 |
BDNF | Rabbit | 1:200 | Bioss | BS-4989R |
HuCD | Mouse | 1:15 | Invitrogen - Thermofisher scientific | A-21271 |
MAP-2 | Rabbit | 1:1000 | Abcam | ab32454 |
PSD 95 | Mouse | 1:200 | Invitrogen | 7E3-1B8 |
Anti-Rabbit IgG (H+L) [FITC] | Donkey | 1:1000 | Novusbio | NB120-6798 |
Biotin-SP (long spacer) AffiniPure F(ab')2 Fragment Anti-Rabbit IgG (H+L) | Donkey | 1:3000 | Jackson ImmunoResearch | 711-066-152 |
Anti-Mouse IgG (H+L) [Texas Red] | Goat | 1:500 | Novusbio | NB120-6787 |