Fig. 4

miR-155 and miR-146a suppress meningitic E. coli PCN033-induced activation of NF-κB signaling. a Western blot analysis of NF-κB p65 and phosphorylated p65 in U251 cells in response to PCN033 infection at different time points. β-actin was used as a loading control. b and c U251 cells were transfected as in Figs. 3a and b and infected with PCN033 at a MOI of 10 for 2 h; the protein levels of NF-κB p65 and phosphorylated p65 were determined by Western blotting (n = 3/group). d U251 cells were transfected with miR-ctrl, miR-155, or miR-146a and infected with PCN033. Translocation of the p65 subunit was detected by fluorescence microscopy. The p65 was labeled in red, and the cell nucleus was stained in blue with DAPI. Scale bar indicated 20 μm