Skip to main content
Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: miR-19a/b-3p promotes inflammation during cerebral ischemia/reperfusion injury via SIRT1/FoxO3/SPHK1 pathway

Fig. 3

Knockdown of FoxO3 ameliorated OGD/R-induced cell death. a Relative FoxO3 protein level in cells transfected with sh-NC or sh-FoxO3. b MTT assay to analyze cell viability in transfected cells following OGD/R. c TUNEL staining to measure the number of apoptotic cells in transfected cells upon OGD/R. d Relative protein levels of SPHK1 and NF-κB p65 in transfected cells following OGD/R. e Relative levels of TNF-α, IL-6, and IL-1β in transfected cells following OGD/R. All the results were shown as mean ± SD (n = 3), which were three different experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.01

Back to article page