Fig. 4From: miR-19a/b-3p promotes inflammation during cerebral ischemia/reperfusion injury via SIRT1/FoxO3/SPHK1 pathwayFoxO3 transcriptionally activated SPHK1 expression. a Relative SPHK1 protein levels in cells transfected with sh-NC or sh-FoxO3. b Predicted binding sites of FoxO3 in SPHK1 promoter. c EMSA to determine the interaction between FoxO3 and SPHK1 promoter. d ChIP to analyze FoxO3/SPHK1 promoter interaction. e Quantification of relative abundance of FoxO3 bound to SPHK1 promoter. All the results were shown as mean ± SD (n = 3), which were three different experiments performed in triplicate. *P < 0.05, ***P < 0.01Back to article page